Pediococcus cerevisiae mutant with altered transport of folates
نویسندگان
چکیده
منابع مشابه
Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.
A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent (Q(10) between 27 C and 37 C was about 2), energy-dependent, and pH-dependent ...
متن کاملTransport of folinate and related compounds in Pediococcus cerevisiae.
The properties of folinate and 5-methyltetrahydrofolate (5-CH(3)-H(4)PteGlu) transport mechanism of Pediococcus cerevisiae were studied. The uptake was dependent on temperature, pH (optimum for both compounds at pH 6.0), and glucose. Iodoacetate, potassium fluoride, and sodium azide inhibited the uptake. 5-CH(3)-H(4)-PteGlu was apparently not metabolized but folinate was metabolized. Metabolism...
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Five parental strains of Pediococcus were examined for plasmid content. Each strain contained three to six resident plasmids, ranging in size from 4.5 to 39.5 megadaltons. A bacteriocin-like substance produced by Pediococcus cerevisiae FBB63 was tentatively linked to a 10.5-megadalton plasmid after being cured with novobiocin.
متن کاملUptake and Metabolism of Folate by a Folate-utilizing Mutant of Pediococcus cerevisiae
Pediococcus cerevisiae 8081, which uses folinic acid for growth, and its nutritional mutant 8081/S, which utilizes folate, were compared with respect to their ability to take up and metabolize folate. In contrast to the parent strain 8081, which takes up very little folate, the uptake of folate by the mutant is a concentrative, saturable, and energydependent process. At low extracellular concen...
متن کاملPhysiological and enzymatic properties of a thymidine-requiring Pediococcus cerevisiae mutant.
We describe the isolation and characterization of a Pediococcus cerevisiae thymidine-requiring mutant and its thymidine-independent revertant. The mutant strain lacked thymidylate synthetase activity and had an absolute requirement for low concentrations (2 micrograms/ml) of thymidine in addition to a requirement for N-5-formyl tetrahydrofolic acid (folinate). Even at high concentrations (up to...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1975
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.123.2.400-406.1975